Trifluoroacetic Acid

Description

Trifluoroacetic acid (TFA) is a specialized reagent supplied in a 500 mL quantity. Its primary application is in biochemical research, particularly within peptide synthesis and sequencing workflows. The compound, also known as perfluoroacetic acid, serves as a strong acid and an effective ion-pairing agent. In chromatography, it is commonly used to modify mobile phases, improving the separation and peak shape of peptides and proteins by suppressing silanol interactions on reversed-phase columns. This reagent is a clear, fuming liquid with a low boiling point and a pungent odor, requiring careful handling in a well-ventilated fume hood. Its properties make it a staple in laboratories focused on proteomics and analytical biochemistry.