Selective Plane Illumination Microscopy
Manufacturer: Applied Scientific Instrumentation
SKU: SPIM
Description
Selective Plane Illumination Microscopy (SPIM), offered by Applied Scientific Instrumentation, is a light sheet fluorescence microscopy technique. This method illuminates only a thin plane of the sample at a time with a sheet of laser light, while a detection objective positioned orthogonally captures the emitted fluorescence. This configuration results in several advantages: rapid volumetric imaging with inherent optical sectioning, significantly reduced photobleaching, and minimal photodamage to living specimens compared to point-scanning techniques like confocal microscopy. ASI provides modular components that allow researchers to configure custom SPIM setups, supporting implementations such as diSPIM, ct-dSPIM, and oSPIM for various experimental needs.
Specifications
| Item | Selective Plane Illumination Microscopy |
| Company | Applied Scientific Instrumentation |
| Price | Inquire |
| Catalog Number | SPIM |
| Quantity | EA |
| Type | Light Sheet Microscopy |
| Pixels | Inquire |
| Exposure Time | Inquire |
| Mount | Cover slip or open dish, Open dish with objectives immersed in media |
| Cooling Method | Inquire |
| Configurations | ASI offers a variety of modular microscope elements to easily implement light sheet microscopes including: Motorized and piezo stages Light sheet generators for either static or scanned sheets Tunable lens for remote focus and translating beam waist All associated control electronics, including coordination of these elements with cameras and lasers Optomechanics, e.g. filter holders, kinematic mirrors, tube lenses Objective for cleared tissue imaging Other modules generally useful for infinity microscopes including hardware autofocus, LED illuminators, turrets and objective changers, automated and manual filter sliders, automated and manual port switchers, filter wheels, etc. The most common configurations of ASI hardware in light sheet microscopes are: SPIM and diSPIM oSPIM ct-dSPIM Others configurations: Zebrafish imaging OpenSPIM geometry with XYZT stage Open-top |