ProteanFect™ TuffCell Transfection Kit

Description

The ProteanFect TuffCell Transfection Kit from OriGene Technologies is a reagent system designed for introducing nucleic acids into cultured cells, with a particular focus on cell types that are traditionally difficult to transfect. This includes primary cells, stem cells, neurons, and immune cells such as T cells and NK cells. The kit utilizes a non-viral, protein-based delivery platform that forms complexes with nucleic acids to facilitate their uptake by cells. This method is promoted as an alternative to lipid-based transfection or electroporation, with the aim of reducing cellular toxicity and preserving normal cell function and viability. The reagents are compatible with various nucleic acid cargo types, including plasmid DNA, mRNA, siRNA, and CRISPR components. The kit is available in multiple sizes to accommodate different experimental scales.