Nexera UC On-line SFE-SFC System

Description

The Nexera UC is an integrated system for online supercritical fluid extraction and supercritical fluid chromatography (SFE-SFC). It combines extraction and analysis into a single, automated workflow. The chromatography component operates with supercritical CO2, offering flow rates from 0.0001 mL/min to 5 mL/min, with maximum pressure capabilities varying by configuration. The system is designed for method development and can be configured to automatically select from a library of up to 12 different columns and create gradients using blends of up to four modifier solvents. This automation of column and solvent switching, including associated equilibration steps, aims to reduce manual intervention and potential for error. Detection options include UV/VIS, photodiode array (PDA), and mass spectrometry (LCMS). The system is controlled by dedicated Method Scouting Solution software, which manages a database of columns and modifiers. Users can select analytical conditions via a graphical interface, and the software assists with calculations for modifier usage, performs post-analysis column rinsing protocols, and can predict analysis completion times. The use of supercritical CO2 as a mobile phase, noted for its low viscosity, can contribute to faster separation speeds compared to some liquid chromatographic methods.