Nexera Mikros Microflow Liquid Chromatography Mass Spectrometer System

Description

The Nexera Mikros is a microflow LC-MS system designed for applications requiring high sensitivity. It operates across a flow rate range from 0.1 µL/min (microflow) up to 500 µL/min (semi-microflow), bridging the gap between nanoflow and conventional UHPLC. A key component is its specifically engineered micro-electrospray ionization (micro-ESI) source, which is reported to provide sensitivity improvements exceeding tenfold compared to standard UHPLC-MS configurations for analyses of small molecules, peptides, and proteins. The system incorporates features to minimize extra-column volume, such as a column oven mounted directly on the LC-MS interface. An adjustable stage with a viewing camera facilitates precise alignment of the ESI spray needle. For column connectivity, the proprietary UF-Link technology is employed to create robust, zero-dead-volume connections with microflow columns. The system includes an autosampler capable of injection volumes from 0.1 µL to 50 µL, noted for low carryover and reproducible performance. The mass spectrometer utilizes molybdenum hyperbolic mass filters for both the Q1 and Q3 quadrupoles, with pre-rods and additional post-rods in Q1.