Hydrophobic Column

Description

This hydrophobic column from G Biosciences is designed for hydrophobic interaction chromatography (HIC), a technique used to separate biomolecules like proteins based on differences in their surface hydrophobicity. The column contains a stationary phase with hydrophobic ligands, which interact with non-polar regions on target molecules under high-salt conditions. As the salt concentration is decreased during elution, molecules are released in order of increasing hydrophobicity. This method is particularly valuable for purifying proteins and other biological macromolecules while maintaining their native structure and biological activity, as it operates under relatively mild, non-denaturing conditions compared to some other chromatographic modes.