Femto Pulse System

Description

The Agilent Femto Pulse System (M5330AA) is a quantitative gel electrophoresis instrument for nucleic acid analysis. It utilizes pulsed-field electrophoresis to separate large DNA fragments, with a resolving capacity for high molecular weight DNA up to 165 kilobases in roughly 90 minutes. A primary technical attribute is its high sensitivity, capable of detecting nucleic acids at concentrations as low as 50 femtograms per microliter. This makes it suitable for analyzing low-concentration samples and nucleic acid smears where conventional gel methods may lack sufficient sensitivity. The system processes 12 samples per run under ambient temperature conditions (20-23°C). Its applications include the analysis of high molecular weight DNA, large DNA fragment libraries, BAC clones, and quality control for samples such as cell-free DNA (cfDNA), genomic DNA, and RNA. The associated workflow is designed to simplify sample preparation using stable reagents, which helps maintain sample integrity. Integrated software automates analysis for different sample types, potentially streamlining workflows like long-read next-generation sequencing library preparation and reducing reliance on traditional pulsed-field gel electrophoresis.