DynaPro™ ZetaStar™ Dynamic, Electrophoretic, and Static Light Scattering Instrument
Manufacturer: Waters | Wyatt Technology
SKU: DynaPro ZetaStar
Description
This instrument combines three analytical light scattering methods—dynamic (DLS), electrophoretic (ELS), and static (SLS)—into one platform for multi-parameter characterization of colloidal systems. It measures particle size and distribution via DLS, zeta potential and electrophoretic mobility via ELS, and molar mass, concentration, and turbidity via SLS. Designed for versatility, it analyzes samples including proteins, polymers, viruses, gene vectors, and various synthetic nanoparticles. Typical measurement times are around 15 seconds. The system supports both automated high-throughput analysis in microplate format and manual cuvette-based operation via its onboard interface. Sample volume requirements are low, with size measurements from 2 µL and zeta potential from approximately 65 µL. A flow-cell mode enhances precision for samples in challenging buffers. The instrument can also be interfaced with HPLC systems for automated fraction analysis.
Specifications
| Item | DynaPro™ ZetaStar™ Dynamic, Electrophoretic, and Static Light Scattering Instrument |
| Company | Waters | Wyatt Technology |
| Catalog Number | DynaPro ZetaStar |
| Measurement Mode(s) | ELS: electrophoretic mobility, zeta potential, electrical chargeDLS: size, size distributions, polydispersitySLS: particle concentration, molar mass, turbidity |
| Applications | Analysis of proteins, gene vectors, viruses and virus-like particles, promiscuous inhibitors, micelles, nano-emulsions, lipid and metallic nanoparticles, and more. |
| Type | Dynamic Light Scattering (DLS), Electrophoretic Light Scattering (ELS), Static Light Scattering (SLS) |
| Measuring Time | 15 seconds typical |
| Sample Type | Proteins, polymers, and nanoparticles including viruses, liposomes, gene vectors, nanoemulsions, etc. |
| Particle Size | 0.2 to 1000 nm |
| Light Source | 785 nm laser, programmable 10% to 100% |
| Pressure Range | Flow cell pressurizable to 400 psi for bubble suppression in conductive solvents |
| Optical System | Multiple optical channels: in-cuvette DLS & SLS, in-cuvette or flow cell DLS & ELS, fiber connection to Wyatt MALS detector for online DLS. ELS optical system: fiber-coupled interferometric Doppler electrophoretic light scattering (FIDELIS), determines electrophoretic mobility and zeta potential from frequency shift of scattered light |
| Linearity | SLS detector is linear over 6 decades |
| Principle | Dynamic Light Scattering (DLS), Electrophoretic Light Scattering (ELS), Static Light Scattering (SLS) |
| Sensitivity | DLS: 0.1 mg/mL of lyozyme; ELS: 1 mg/mL BSA; SLS: 0.1 mg/mL lysozyme |
| Measuring Range | Size: 0.2nm to 1000nm (hydrodynamic radius); Zeta potential: No practical limit; Molecular weight: 300Da to 1MDa |
| Wavelength(s) | 785 nm |
| Detection System | DLS: Avalanche photodiode at 90° or 163.5° scattering angle SLS: photodiode at 90° or 163.5° scattering angleELS Detection: photodiode at 10° scattering angle for FIDELIS (fiber-interferometric Doppler electrophoretic light scattering) |
| Sample Volume | Quartz Cuvette: 2µL and 45µL PEEK Flow Cell: 350µL Dip Cell: 65µL Disposable DLS Cuvette: 4µL Disposable Zeta Cell: 400µL |
| Dimensions | (WxDxH) 36 x 57 x 20 cm |
| Temperature Range | -10 °C to +120 °C |
| Interface | DYNAMICS TOUCH onboard app or DYNAMICS Software on PC |
| Control | Software control of detector and autosampler system to automate a series of unattended measurements |