DAWN™ Multi-angle Light Scattering (MALS) Instrument
Manufacturer: Waters | Wyatt Technology
SKU: DAWN
Description
The DAWN instrument, from Wyatt Technology (a Waters company), is a multi-angle light scattering (MALS) detector used for the absolute characterization of macromolecules and nanoparticles. It measures scattered light intensity at 18 discrete angles using high-gain, high-dynamic-range photodiodes. The standard light source is a 658 nm laser with programmable power output from 10% to 100%; an optional 785 nm laser is available to minimize fluorescence interference from certain samples. The instrument determines absolute molar mass and size (radius of gyration) without the need for column calibration. An optional dynamic light scattering (DLS) module can be added to measure hydrodynamic radius. Its effective measurement range extends from 200 Daltons to 1,000 Megadaltons. It is routinely used in conjunction with separation techniques like size-exclusion chromatography (SEC-MALS) or field-flow fractionation (FFF-MALS). Operation and data analysis are conducted through the ASTRA software suite, and a touch-screen interface provides real-time diagnostics. Applications include the analysis of proteins, polymers, viral vectors, and lipid nanoparticles.
Specifications
| Item | DAWN™ Multi-angle Light Scattering (MALS) Instrument |
| Company | Waters | Wyatt Technology |
| Catalog Number | DAWN |
| Quantity | EA |
| Type | MALS and optional DLS |
| Detector(s) | High-gain, high-dynamic-range photodiodes at 18 angles for MALS; optional avalanche photodiode that may be placed at any of four angles for DLS. Fluorescence-blocking filters available for use with analytes that fluoresce when illuminated at the MALS wavelength. |
| Light Source | 658 nm laser, programmable 10% - 100% of maximum power. Optional: 785 nm for fluorescence suppression |
| Measuring Range | 200 Da to 1000 MDa |
| Wavelength(s) | 658 nm; 785 nm available for fluorescence reduction (with lower sensitivity than 658 nm) |
| Control | Programmable 10% - 100% (laser power) |
| Temperature Range | Ambient; Heated/Cooled (HC): -15°C to +150°C; Ultra-High-Temperature (UHT): ambient to +210°C |
| Radius of Gyration Range | Rg (rms radius): 10 nm to 500 nmRh(hydrodynamic radius): 0.5 nm to 300 nm or up to 1 µM (with optional DLS module, upper limit configuration-dependent) |
| Dimensions | (WxDxH) 36 x 60 x 23 cm |
| Dynamic Range | 3,300,000:1 |
| Sensitivity | 10 ng of 100 kDa polystyrene in THF, under typical GPC conditions0.2 µg of BSA under typical 30 cm column |
| Interface | 10" high-resolution, color LCD multi-touch-screen display, and ASTRA host software |
| Measuring Time | Depedent on LC separation |
| Applications | With standard SEC/GPC or EclipseTM (FFF system): determination of distributions of molar mass, size, conformation, conjugation ratio and particle concentration. With Calypso composition-gradient system: label-free characterization of biomolecular interactions including self- and hetero-association as well as non-specific interactions. |
| Sample Type | Peptides, proteins, nucleic acids, polymers, lipid nanoparticles, viruses, and other macromolecules |
| Measurement Mode(s) | 18 angles |