Biometra TRobot II – Automated Thermal Cycler

Description

The Biometra TRobot II from Analytik Jena is an automated thermal cycler designed for precise temperature control in microplate-based molecular biology applications. While fully capable of standard PCR, its functionality extends to various incubation steps requiring accurate thermal management, such as those used in Next Generation Sequencing (NGS) library preparation. The instrument is characterized by rapid heating and cooling rates and provides high temperature uniformity across its sample block, which is critical for reproducible results. It is compatible with 96-well and 384-well plate formats. The unit includes a heated lid with an adjustable temperature range from 30 °C to 110 °C. A key feature for protocol optimization is its gradient capability, which allows a defined temperature range to be tested across different rows of the block in a single run. This is particularly useful for determining optimal annealing temperatures when establishing new PCR protocols. The gradient function can be applied over 12 or 24 rows, depending on the specific model, with a typical gradient temperature range from 10 °C to 99 °C. The overall instrument temperature range is 3 °C to 99 °C.