Annexin V-PE Apoptosis Detection Kit

Description

The Annexin V-PE Apoptosis Detection Kit from MedChemExpress (Catalog HY-K1075) is designed for the identification of apoptotic and necrotic cells in a sample. This assay kit utilizes Annexin V conjugated to phycoerythrin (PE), a fluorescent dye, to bind to phosphatidylserine (PS). In healthy cells, PS is located on the inner leaflet of the plasma membrane, but during early apoptosis, it translocates to the outer surface, where it becomes accessible for Annexin V-PE binding. The kit provides a method to distinguish between live cells (showing minimal fluorescence), apoptotic cells (displaying red fluorescence from bound Annexin V-PE), and necrotic cells (which may also show fluorescence due to membrane permeability). Available in 20-test and 50-test sizes, it offers a standardized protocol for flow cytometry or fluorescence microscopy applications in cell biology research.