Affinity Isothermal Titration Calorimeter

Description

The Affinity Isothermal Titration Calorimeter, manufactured by TA Instruments – Waters LLC, is an analytical instrument used to directly measure the heat change during a biochemical or chemical binding reaction. By maintaining a constant temperature—operable within a range of 2 to 80°C—it quantifies the enthalpy, entropy, stoichiometry, and binding affinity (equilibrium constant) of molecular interactions in a single experiment. This technique is label-free and solution-based, making it applicable for studying protein-ligand binding, protein-protein interactions, antibody-antigen affinity, and small molecule drug discovery. The instrument provides a complete thermodynamic profile, offering insights into the driving forces behind molecular association that other methods may not capture.