3-Phosphoglycerate Kinase (PGK) Activity Assay Kit (UV Colorimetric Method)

Description

This assay kit from Aladdin Scientific Corporation provides reagents for the quantitative measurement of 3-phosphoglycerate kinase (PGK) activity using a UV colorimetric method. PGK is an essential glycolytic enzyme that catalyzes the transfer of a phosphate group from ATP to 3-phosphoglycerate, forming 1,3-bisphosphoglycerate. The kit's principle relies on the subsequent enzymatic conversion of this product by glyceraldehyde-3-phosphate dehydrogenase, with a concomitant decrease in NADH absorbance measured at 340 nm. The supplied format is liquid, with a quantity sufficient for 50 tests (50T). Components include an extraction buffer and multiple reagent vials, each with specific storage requirements, typically at 2-8°C or -20°C. The protocol details sample extraction from tissues, bacterial cultures, or cell lines, followed by a spectrophotometric assay procedure. Calculations for enzyme activity are provided based on sample mass, protein concentration, or cell count. Users are advised to perform preliminary tests to optimize sample amounts and are directed to compatible protein quantification kits for accurate normalization.